Facilities
The DBT Platform are state of art covering various specialty and frontier areas of research like Genomics, Proteomics, Molecular Platforms, Bio-Imaging and SPF Animal facility with many sophisticated instruments. This enables DBT to productively conduct its research as well as bring immense value to any counterpart research collaborator.
Genomics Facility
NextSeq 500
NextSeq 500 works on Illumina’s proven Sequencing by synthesis (SBS) technology
NextSeq 500
NextSeq 500 works on Illumina’s proven Sequencing by synthesis (SBS) technology which enables rapid sequencing of whole genomes, exomes, targeted panels, and transcriptomes in a single run. With SBS v2 chemistry, the NextSeq Series provides exceptional accuracy at any coverage level. The NextSeq 500 instrument is capable of generating read lengths as follows: single-end 75 bp, single-end 150 bp, paired-end 2 x 75 bp, and paired-end 2 x 150 bp.
Services Offered
- Sanger sequencing - Seqstudio Instrument
- Whole Genome Sequencing
- Whole Exome sequencing
- Exome and Targeted sequencing
- Metagenomics
- Whole RNA / Transcriptome sequencing
- Ribosome profiling
- DNA methylation sequencing
- ChIP - Sequencing
Proteomics Facility
Bruker MALDI-TOF (UltrafleXtreme II)
The enhanced ultrafleXtreme incorporates next-generation MALDI-TOF and TOF/TOF technology.
Bruker MALDI-TOF (UltrafleXtreme II)
The enhanced ultrafleXtreme incorporates next-generation MALDI-TOF and TOF/TOF technology. The innovative smartbeam-II™ laser enables
ultra-high data acquisition speed in both MS and MS/MS
Services Offered
- Mass measurement of Proteins, Peptides, Oligonucleotides & Other molecules (Organic & Inorganic).
Molecular Platforms
QuantStudio 3D Digital PCR &
QuantStudio 5 Real-Time PCR System
The QuantStudio® 3D Digital PCR System produces absolute quantification data with precision and sensitivity. Its applications include rare mutation detection, pathogen detection, viral load quantification, genetically modified organism detection etc. The QuantStudio™ 5 Real-Time PCR System features VeriFlex™ technology which offers 6 independent temperature zones for PCR optimization. Inbuilt OptiFlex technology provides enhanced fluorescence detection enabling accurate and sensitive data analysis with multiplexing capacity of up to 6 targets.
Services Offered
- Genotyping/Allelic Discrimination
- Genetically Modified Organism (GMO) Detection
- Gene Expression Analysis
- Molecular Oncology (Detection of hotspot and fusion mutations)
- Pathogen detection
- Liquid biopsy
- Copy number variation (CNV)
- Rare sequence detection
- Rare mutation detection
- Gene expression and miRNA analysis
QuantStudio 3D Digital PCR and QuantStudio 5 Real-Time PCR System
Microarray
The Agilent Microarray Scanner is a laser induced fluorescence scanner
Microarray
The Agilent Microarray Scanner is a laser induced fluorescence scanner designed to read microarrays printed on a standard 1 inch x 3 inch glass slides. The SureScan scanner measures the fluorescence intensity of
labeled sample nucleic acid (DNA and RNA) bound to microarrays. Its ability to measure fluorescence from two dyes simultaneously facilitates all two-color microarray studies. The dyes compatible with the system are Cyanine 3 and Cyanine 5, and Alexa 647, 555, and 660.
Services Offered
- DNA Microarrays
- CGH & CGH+SNP Microarrays
- CNV Microarrays
- DNA Methylation Microarrays
- ChiP-on-ChiP Microarrays
- Exon Microarrays
- RNA Microarrays
- miRNA Microarrays
Bioplex 200
Bioplex 200 is a multiplex ELISA system based on Luminex’s xMAP technology. By combining advanced fluidics, optics, and digital signal processing with proprietary microsphere (“bead”) technology, xMAP Technology enables a high degree of multiplexing within a single sample volume. The system has capacity to detect upto 100 different analytes from a single sample in a single reaction well.
Services Offered
- Inflammation assays
- Diabetes, Metabolic and Hormone assays
- Cytokine, Chemokine and Growth factor assays
- Cell signaling assays
- Toxicity assays
- Cancer biomarker and disease research assays
- Customized bead based multiplex ELISA
Bioplex 200
Bioplex 200 is a multiplex ELISA system based on Luminex’s xMAP technology.
Bio-Imaging Facility
Leica DM6 Fluorescent Microscope with Cryostat
Leica DM6 Fluorescent Microscope with Cryostat
The Leica DM6 is an upright digital research microscope with 19 mm sCMOS camera imaging port that can easily capture quality images. Automated Koehler light management ensures that images are publication ready. The Fluorescence Intensity Manager (FIM) provides fast and reproducible regulation of the excitation light to effectively protect the specimen from photo bleaching.
Services Offered
- Tissue sectioning
- Slide Visualization
